Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.

ABSTRACT:

Introduction

SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca²⁺) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).

Methods

Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.

Results

SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, an

SUBMITTER: Ba G 

PROVIDER: S-EPMC8127738 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature