Oxidative stress induces cell death partially by decreasing both mRNA and protein levels of nicotinamide phosphoribosyltransferase in differentiated PC12 cells.

ABSTRACT:

Background

Multiple studies have indicated crucial roles of NAD⁺ deficiency in several neurological diseases and aging. It is critical to discover the mechanisms underlying the NAD⁺ deficiency. A decreased level of Nicotinamide phosphoribosyltransferase (Nampt)-an important enzyme in the salvage pathway of NAD⁺ synthesis-has been found under certain pathological conditions, while the mechanisms underlying the Nampt decrease are unclear. The purpose of this study is to test the hypothesis that oxidative stress can produce decreased Nampt, and to investigate the biological effects of Nampt on NAD⁺ synthesis and cell survival under both basal and oxidative stress conditions.

Methods

We used differentiated PC12 cells as a cellular mode

SUBMITTER: Zhou C 

PROVIDER: S-EPMC8127959 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature