A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects.

ABSTRACT: Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and i

SUBMITTER: Perez M 

PROVIDER: S-EPMC8128205 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature