Differential expression profiles of miRNAs and their putative targets in Schistosoma mansoni during its life cycle.

ABSTRACT:

Background

Schistosomiasis is a disease caused by Schistosoma. Due to its complex life cycle, evolutionary position and sexual dimorphism, schistosomes have several mechanisms of gene regulation. MicroRNAs (miRNAs) are short endogenous RNAs that regulate gene expression at the post-transcriptional level by targeting mRNA transcripts.

Objectives

Here, we tested 12 miRNAs and identified their putative targets using a computational approach.

Methods

We performed the expression profiles of a set of miRNAs and their putative targets during the parasite's life cycle by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Findings

Our results showed differential expression patterns of the mature miRNAs sma-miR-250; sma-miR-92a; sma-miR-new_4-3p; sma

SUBMITTER: Abreu FC 

PROVIDER: S-EPMC8128373 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature