Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models.

ABSTRACT: Tissues are complex mixtures of different cell subtypes, and this diversity is increasingly characterized using high-throughput single cell analysis methods. However, these efforts are hindered, as tissues must first be dissociated into single cell suspensions using methods that are often inefficient, labor-intensive, highly variable, and potentially biased towards certain cell subtypes. Here, we present a microfluidic platform consisting of three tissue processing technologies that combine tissue digestion, disaggregation, and filtration. The platform is evaluated using a diverse array of tissues. For kidney and mammary tumor, microfluidic processing produces 2.5-fold more single cells. Single cell RNA sequencing further reveals that endothelial cells, fibroblasts, and basal epithelium ar

SUBMITTER: Lombardo JA 

PROVIDER: S-EPMC8128882 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature