Characterization of a new bifunctional endo-1,4-β-xylanase/esterase found in the rumen metagenome.

ABSTRACT: Metagenomic data mining of the Nellore cattle rumen microbiota identified a new bifunctional enzyme, endo-1,4-β-xylanase/esterase, which was subsequently overexpressed in E. coli BL21 (DE3). This enzyme was stable at pH intervals of 5 to 6.5 and temperatures between 30 and 45 °C, and under the test conditions, it had a V_max of 30.959 ± 2.334 µmol/min/mg, K_m of 3.6 ± 0.6 mM and k_cat of 2.323 ± 175 s^-1. Additionally, the results showed that the enzyme is tolerant to NaCl and organic solvents and therefore is suitable for industrial environments. Xylanases are widely applicable, and the synergistic activity of endo-1,4-β-xylanase/esterase in a single molecule will improve the degradation efficiency of heteroxylans via the creation of xylanase bindin

SUBMITTER: Pavarina GC 

PROVIDER: S-EPMC8128909 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature