Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs.

ABSTRACT: Aggregation and self-sorting of cells in three dimensional cultures have been described for non-neuronal cells. Despite increased interest in engineered neural tissues for treating brain injury or for modeling neurological disorders in vitro, little data is available on collective cell movements in neuronal aggregates. Migration and sorting of cells may alter these constructs' morphology and, therefore, the function of their neural circuitry. In this work, linear, adhered rat and human 3D neuronal-astrocyte cultures were developed to enable the study of aggregation and sorting of these cells. An in silico model of the contraction, clustering, and cell sorting in the 3D cultures was also developed. Experiments and computational modeling showed that aggregation was mainly a neuron mediated p

SUBMITTER: Hasan MF 

PROVIDER: S-EPMC8129100 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature