SIRT3 overexpression and epigenetic silencing of catalase regulate ROS accumulation in CLL cells activating AXL signaling axis.

ABSTRACT: Mitochondrial metabolism is the key source for abundant ROS in chronic lymphocytic leukemia (CLL) cells. Here, we detected significantly lower superoxide anion (O₂^-) levels with increased accumulation of hydrogen peroxide (H₂O₂) in CLL cells vs. normal B-cells. Further analysis indicated that mitochondrial superoxide dismutase (SOD)2, which converts O₂^- into H₂O₂ remained deacetylated in CLL cells due to SIRT3 overexpression resulting its constitutive activation. In addition, catalase expression was also reduced in CLL cells suggesting impairment of H₂O₂-conversion into water and O₂ which may cause H₂O₂-accumulation. Importantly, we identified two C

SUBMITTER: Maiti GP 

PROVIDER: S-EPMC8129117 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature