Dataset Information

Transportation container for pre-processing cytogenetic assays in radiation accidents.

ABSTRACT: We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson's correlation coefficient: 0.992). The container's ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.

SUBMITTER: Gu J

PROVIDER: S-EPMC8129553 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Transportation container for pre-processing cytogenetic assays in radiation accidents.

Gu Jian J Duane Brett B Repin Mikhail M Brenner David J DJ Zenhausern Frederic F

Scientific reports 20210517 1

We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay us ...[more]

PMID: 34001964

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Project description:BackgroundRadiation overexposure accidents are rare but can have severe long-term health consequences. Although underreporting can be an issue, some extensive literature reviews of reported radiation overexposures have been performed and constitute a sound basis for conclusions on general trends. Building further on this work, we performed a systematic review that completes previous reviews and provides new information on characteristics and trends of reported radiation accidents.MethodsWe searched publications and reports from MEDLINE, EMBASE, the International Atomic Energy Agency, the International Radiation Protection Association, the United Nations Scientific Committee on the Effects of Atomic Radiation, the United States Nuclear Regulatory Commission, and the Radiation Emergency Assistance Center/Training Site radiation accident registry over 1980-2013. We retrieved the reported overexposure cases, systematically extracted selected information, and performed a descriptive analysis.Results297 out of 5189 publications and reports and 194 records from the REAC/TS registry met our eligibility criteria. From these, 634 reported radiation accidents were retrieved, involving 2390 overexposed people, of whom 190 died from their overexposure. The number of reported cases has decreased for all types of radiation use, but the medical one. 64% of retrieved overexposure cases occurred with the use of radiation therapy and fluoroscopy. Additionally, the types of reported accidents differed significantly across regions.ConclusionsThis review provides an updated and broader view of reported radiation overexposures. It suggests an overall decline in reported radiation overexposures over 1980-2013. The greatest share of reported overexposures occurred in the medical fields using radiation therapy and fluoroscopy; this larger number of reported overexposures accidents indicates the potential need for enhanced quality assurance programs. Our data also highlights variations in characteristics of reported accidents by region. The main limitation of this study is the likely underreporting of radiation overexposures. Ensuring a comprehensive monitoring and reporting of radiation overexposures is paramount to inform and tailor prevention interventions to local needs.

Project description:IntroductionNowadays, Injuries, disabilities, and deaths due to road traffic accidents pose a major threat to public health worldwide. There was no meta-analysis study conducted in this area in Africa, hence the need for the study.ObjectivesThis review aimed to assess the pooled prevalence and associated factors of road traffic accidents among public transportation in Africa.MethodsThis systematic review and meta-analysis study was conducted in Africa according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Using Boolean logic operators and targeted keywords, we searched for publications on several electronic databases (Web of Science, PubMed, Google Scholar, African Journals Online (AJOL), and Science Direct). The degree of heterogeneity among the included studies, the 95% confidence interval, and the pooled prevalence were estimated using a random effects model.ResultsThis review included 45 studies with 15,968 participants. The overall estimated pooled prevalence of road traffic accident among public transportation in Africa was found to be 38.83% (95% CI: 33.54, 44.12). Meta-regression analysis indicated that sample size, publication year, country, study design, and sub-region had no significant impact on RTA prevalence. Based on a sub-group analysis by countries where the studies were conducted, the higher pooled prevalence of road traffic accidents was found in Congo at 67.90% (95% CI: 59.99, 75.81), while the lower pooled prevalence of road traffic accidents was in Libya at 15.80% (95% CI: 10.99, 20.61). Driving experience (AOR: 2.64, 95% CI: 1.70, 3.34), chewing Khat while driving (AOR = 4.69, CI: 2.80, 7.85), alcohol use (AOR = 2.72, CI: 1.49, 4.97), and receiving mobile phone calls while driving (AOR = 2.37, CI: 1.42, 3.95) were factors significantly associated with road traffic accidents.ConclusionIn conclusion, the pooled prevalence of road traffic accidents in Africa was found to be high. Therefore, we recommend that drivers have to strictly adhere to traffic regulations Moreover, It is recommended that policymakers and administrators ought to gain awareness of road traffic accidents and its risk factors to put existing road traffic accidents preventive and control measures into action.

Dataset Information

Transportation container for pre-processing cytogenetic assays in radiation accidents.

Publications

Transportation container for pre-processing cytogenetic assays in radiation accidents.

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