Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping.

ABSTRACT: N⁴-acetylcytidine (ac⁴C) is an ancient and highly conserved RNA modification that is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA^1-3. However, the distribution, dynamics and functions of cytidine acetylation have yet to be fully elucidated. Here we report ac⁴C-seq, a chemical genomic method for the transcriptome-wide quantitative mapping of ac⁴C at single-nucleotide resolution. In human and yeast mRNAs, ac⁴C sites are not detected but can be induced-at a conserved sequence motif-via the ectopic overexpression of eukaryotic acetyltransferase complexes. By contrast, cross-evolutionary profiling revealed unprecedented levels of ac⁴C across hundreds of residues in rRNA, tRNA, non-codi

SUBMITTER: Sas-Chen A 

PROVIDER: S-EPMC8130014 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature