Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes.

ABSTRACT:

Background

The purpose of this study was to develop a reliable DNA extraction protocol to use on individual Teladorsagia circumcincta nematode specimens to produce high quality DNA for genome sequencing and phylogenetic analysis. Pooled samples have been critical in providing the groundwork for T. circumcincta genome construction, but there is currently no standard method for extracting high-quality DNA from individual nematodes. 11 extraction kits were compared based on DNA quality, yield, and processing time.

Results

11 extraction protocols were compared, and the concentration and purity of the extracted DNA was quantified. Median DNA concentration among all methods measured on NanoDrop 2000™ ranged between 0.45-11.5 ng/μL, and on Qubit™ ranged between undetectable - 0.962

SUBMITTER: Sloan S 

PROVIDER: S-EPMC8130168 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature