KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription.

ABSTRACT:

Background

As an H3K27me3 demethylase and counteracts polycomb-mediated transcription repression, KDM6B has been implicated in the development and malignant progression in various types of cancers. However, its potential roles in esophageal squamous cell carcinoma (ESCC) have not been explored.

Methods

The expression of KDM6B in human ESCC tissues and cell lines was examined using RT-qPCR, immunohistochemical staining and immunoblotting. The effects of KDM6B on the proliferation and metastasis of ESCC were examined using in vitro and in vivo functional tests. RNA-seq and ChIP-seq assay were used to demonstrate the molecular biological mechanism of KDM6B in ESCC.

Results

We show that the expression level of KDM6B increased significantly in patients with lymph node meta

SUBMITTER: Qin M 

PROVIDER: S-EPMC8130268 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature