A novel autophagy-related genes prognostic risk model and validation of autophagy-related oncogene VPS35 in breast cancer.

ABSTRACT:

Background

Accumulating evidence implies that autophagy plays a critical role in breast cancer development and progression. It is crucial to screen out autophagy-related encoding genes (ARGs) with prognostic value in breast cancer and reveal their biological properties in the aggressiveness of breast cancer.

Methods

Univariate and multivariate Cox proportional hazards analyses were used to identify a prognostic risk model of ARGs from The Cancer Genome Atlas (TCGA). Kaplan-Meier analysis, univariate and multivariate Cox regression analyses and receiver operating characteristic (ROC) curve analysis were performed to validate the risk model. Western blot and immunohistochemistry (IHC) were conducted to assess the expression of VPS35 (one of ARGs in risk model). CCK8, Colony fo

SUBMITTER: Li X 

PROVIDER: S-EPMC8130280 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature