Worenine reverses the Warburg effect and inhibits colon cancer cell growth by negatively regulating HIF-1α.

ABSTRACT:

Background

Some natural compounds inhibit cancer cell growth in various cancer cell lines with fewer side effects than traditional chemotherapy. Here, we explore the pharmacological effects and mechanisms of worenine (isolated from Coptis chinensis) against colorectal cancer.

Methods

The effects of worenine on colorectal cancer cell proliferation, colony formation and cell cycle distribution were measured. Glycolysis was investigated by examining glucose uptake and consumption, lactate production, and the activities and expressions of glycolysis enzymes (PFK-L, HK2 and PKM2). HIF-1α was knocked down and stimulated in vitro to investigate the underlying mechanisms.

Results

Worenine somewhat altered the glucose metabolism and glycolysis (Warburg effect) of cancer cells.

SUBMITTER: Ji L 

PROVIDER: S-EPMC8130299 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature