MTOR controls endoplasmic reticulum-Golgi apparatus trafficking of VSVg in specific cell types.

ABSTRACT:

Background

Mammalian/mechanistic target of rapamycin (mTOR) complexes are essential for cell proliferation, growth, differentiation, and survival. mTORC1 hyperactivation occurs in the tuberous sclerosis complex (TSC). mTORC1 localizes to the surface of lysosomes, where Rheb activates it. However, mTOR was also found on the endoplasmic reticulum (ER) and Golgi apparatus (GA). Recent studies showed that the same inputs regulate ER-to-GA cargo transport and mTORC1 (e.g., the level of amino acids or energy status of the cell). Nonetheless, it remains unknown whether mTOR contributes to the regulation of cargo passage through the secretory pathway.

Methods

The retention using selective hooks (RUSH) approach was used to image movement of model cargo (VSVg) between the ER and GA in

SUBMITTER: Koscielny A 

PROVIDER: S-EPMC8130434 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature