Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes.

ABSTRACT: Transepithelial/transendothelial electrical resistance (TEER) measurements can be applied in organ-on-chips (OoCs) to estimate the barrier properties of a tissue or cell layer in a continuous, non-invasive, and label-free manner. Assessing the barrier integrity in in vitro models is valuable for studying and developing barrier targeting drugs. Several systems for measuring the TEER have been shown, but each of them having their own drawbacks. This article presents a cleanroom-free fabrication method for the integration of platinum electrodes in a polydimethylsiloxane OoC, allowing the real-time assessment of the barrier function by employing impedance spectroscopy. The proposed method and electrode arrangement allow visual inspection of the cells cultured in the device at the site of the e

SUBMITTER: Bossink EGBM 

PROVIDER: S-EPMC8130670 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature