The effect of mitochondrial calcium uniporter and cyclophilin D knockout on resistance of brain mitochondria to Ca²⁺-induced damage.

ABSTRACT: The mitochondrial calcium uniporter (MCU) and cyclophilin D (CyD) are key players in induction of the permeability transition pore (PTP), which leads to mitochondrial depolarization and swelling, the major signs of Ca²⁺-induced mitochondrial damage. Mitochondrial depolarization inhibits ATP production, whereas swelling results in the release of mitochondrial pro-apoptotic proteins. The extent to which simultaneous deletion of MCU and CyD inhibits PTP induction and prevents damage of brain mitochondria is not clear. Here, we investigated the effects of MCU and CyD deletion on the propensity for PTP induction using mitochondria isolated from the brains of MCU-KO, CyD-KO, and newly created MCU/CyD-double knockout (DKO) mice. Neither deletion of MCU nor of CyD affected respiration o

SUBMITTER: Hamilton J 

PROVIDER: S-EPMC8131324 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature