In-depth transcriptomic analysis of human retina reveals molecular mechanisms underlying diabetic retinopathy.

ABSTRACT: Diabetic Retinopathy (DR) is among the major global causes for vision loss. With the rise in diabetes prevalence, an increase in DR incidence is expected. Current understanding of both the molecular etiology and pathways involved in the initiation and progression of DR is limited. Via RNA-Sequencing, we analyzed mRNA and miRNA expression profiles of 80 human post-mortem retinal samples from 43 patients diagnosed with various stages of DR. We found differentially expressed transcripts to be predominantly associated with late stage DR and pathways such as hippo and gap junction signaling. A multivariate regression model identified transcripts with progressive changes throughout disease stages, which in turn displayed significant overlap with sphingolipid and cGMP-PKG signaling. Combined anal

SUBMITTER: Becker K 

PROVIDER: S-EPMC8131353 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature