Genetic pathways regulating hematopoietic lineage speciation: Factorial latent variable model analysis of single cell transcriptome.

ABSTRACT: Genetic pathways regulating hematopoietic lineage commitment at critical stages of development remain incompletely characterized.  To better delineate genetic sources of variability regulating cellular speciation during steady-state hematopoiesis, we applied a factorial single-cell latent variable model (f-scLVM) to decompose single-cell transcriptome heterogeneity into interpretable biological factors (refined pathway annotations or gene sets without annotation) dynamically regulating cell fate.  Hematopoietic single cell transcriptomic raw sequencing data extracted from 1,920 hematopoietic stem and progenitor cells (HSPCs) derived from 12-week-old female mice were used for data analysis and model development. These single cell RNA sequencing data were subsequently analyzed using the fact

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8131567 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature