Ontology highlight
ABSTRACT:
SUBMITTER: Shintomi K
PROVIDER: S-EPMC8131626 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
Shintomi Keishi K Hirano Tatsuya T
Nature communications 20210518 1
Topoisomerase II (topo II) is one of the six proteins essential for mitotic chromatid reconstitution in vitro. It is not fully understood, however, mechanistically how this enzyme regulates this process. In an attempt to further refine the reconstitution assay, we have found that chromosomal binding of Xenopus laevis topo IIα is sensitive to buffer conditions and depends on its C-terminal domain (CTD). Enzymological assays using circular DNA substrates supports the idea that topo IIα first resol ...[more]