The in vitro micronucleus assay using imaging flow cytometry and deep learning.

ABSTRACT: The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity. The MN assay is performed in two variations: scoring MN in cytokinesis-blocked binucleated cells or directly in unblocked mononucleated cells. Several methods have been developed to score the MN assay, including manual and automated microscopy, and conventional flow cytometry, each with advantages and limitations. Previously, we applied imaging flow cytometry (IFC) using the ImageStream^® to develop a rapid and automated MN assay based on high throughput image capture and feature-based image analysis in the IDEAS^® software. However, the analysis strategy required rigorous optimization

SUBMITTER: Rodrigues MA 

PROVIDER: S-EPMC8131758 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature