Engineered Matrices Enable the Culture of Human Patient-Derived Intestinal Organoids.

ABSTRACT: Human intestinal organoids from primary human tissues have the potential to revolutionize personalized medicine and preclinical gastrointestinal disease models. A tunable, fully defined, designer matrix, termed hyaluronan elastin-like protein (HELP) is reported, which enables the formation, differentiation, and passaging of adult primary tissue-derived, epithelial-only intestinal organoids. HELP enables the encapsulation of dissociated patient-derived cells, which then undergo proliferation and formation of enteroids, spherical structures with polarized internal lumens. After 12 rounds of passaging, enteroid growth in HELP materials is found to be statistically similar to that in animal-derived matrices. HELP materials also support the differentiation of human enteroids into mature intesti

SUBMITTER: Hunt DR 

PROVIDER: S-EPMC8132048 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature