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Spatial multi-omics sequencing for fixed tissue via DBiT-seq.

ABSTRACT: This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted panel of proteins. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

SUBMITTER: Su G

PROVIDER: S-EPMC8132129 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Spatial multi-omics sequencing for fixed tissue via DBiT-seq.

Su Graham G Qin Xiaoyu X Enninful Archibald A Bai Zhiliang Z Deng Yanxiang Y Liu Yang Y Fan Rong R

STAR protocols 20210511 2

This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted pa ...[more]

PMID: 34027489

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