Rescuing key native traits in cultured dermal papilla cells for human hair regeneration.

ABSTRACT:

Introduction

The dermal papilla (DP) represents the major regulatory entity within the hair follicle (HF), inducing hair formation and growth through reciprocal interactions with epithelial cells. However, human DP cells rapidly lose their hair inductive ability when cultured in an epithelium-deficient environment.

Objectives

To determine if the conditioned medium collected from interfollicular keratinocytes (KCs-CM) is capable of improving DP cell native properties and inductive phenotype.

Methods

DP cells were cultured with KCs-CM both in 2D and 3D culture conditions (spheroids). Further, the hair-inductive capacity of DP cells precultured with KCs-CM was tested in a hair reconstitution assay, after co-grafting with human keratinocytes in nude mice.

Results

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SUBMITTER: Abreu CM 

PROVIDER: S-EPMC8132206 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature