MultiSero: open multiplex-ELISA platform for analyzing antibody responses to SARS-CoV-2 infection.

ABSTRACT: Serology has provided valuable diagnostic and epidemiological data on antibody responses to SARS-CoV-2 in diverse patient cohorts. Deployment of high content, multiplex serology platforms across the world, including in low and medium income countries, can accelerate longitudinal epidemiological surveys. Here we report multiSero, an open platform to enable multiplex serology with up to 48 antigens in a 96-well format. The platform consists of three components: ELISA-array of printed proteins, a commercial or home-built plate reader, and modular python software for automated analysis (pysero). We validate the platform by comparing antibody titers against the SARS-CoV-2 Spike, receptor binding domain (RBD), and nucleocapsid (N) in 114 sera from COVID-19 positive individuals and 87 pre-pandemi

SUBMITTER: Byrum JR 

PROVIDER: S-EPMC8132273 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature