A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants.

ABSTRACT: Morphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of ∼200 such Caenorhabditis elegans mutants, we have identified 17 that have highly penetrant embryonic morphogenesis defects after upshifts from the permissive to the restrictive temperature, just prior to the cell shape changes that mediate elongation of the ovoid embryo into a vermiform larva. Using whole genome sequencing, we ident

SUBMITTER: Jud MC 

PROVIDER: S-EPMC8133775 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature