ATP hydrolysis and nucleotide exit enhance maltose translocation in the MalFGK₂E importer.

ABSTRACT: ATP binding cassette (ABC) transporters employ ATP hydrolysis to harness substrate translocation across membranes. The Escherichia coli MalFGK₂E maltose importer is an example of a type I ABC importer and a model system for this class of ABC transporters. The MalFGK₂E importer is responsible for the intake of malto-oligossacharides in E.coli. Despite being extensively studied, little is known about the effect of ATP hydrolysis and nucleotide exit on substrate transport. In this work, we studied this phenomenon using extensive molecular dynamics simulations (MD) along with potential of mean force calculations of maltose transport across the pore, in the pre-hydrolysis, post-hydrolysis and nucleotide-free states. We concluded that ATP hydrolysis and nucleotide exit trig

SUBMITTER: Abreu B 

PROVIDER: S-EPMC8134467 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature