Human delta like 1-expressing human mesenchymal stromal cells promote human T cell development and antigen-specific response in humanized NOD/SCID/IL-2R[Formula: see text]^null (NSG) mice.

ABSTRACT: Human delta-like 1 (hDlk1) is known to be able to regulate cell fate decisions during hematopoiesis. Mesenchymal stromal cells (MSCs) are known to exhibit potent immunomodulatory roles in a variety of diseases. Herein, we investigated in vivo functions of hDlk1^-hMSCs and hDlk1⁺hMSCs in T cell development and T cell response to viral infection in humanized NOD/SCID/IL-2Rγ^null (NSG) mice. Co-injection of hDlk1^-hMSC with hCD34⁺ cord blood (CB) cells into the liver of NSG mice markedly suppressed the development of human T cells. In contrast, co-injection of hDlk1⁺hMSC with hCD34⁺ CB cells into the liver of NSG dramatically promoted the development of human T cells. Human T cells developed in humanized NSG mice repres

SUBMITTER: Kwon DH 

PROVIDER: S-EPMC8134586 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature