Experimental virus evolution in cancer cell monolayers, spheroids, and tissue explants.

ABSTRACT: Viral laboratory evolution has been used for different applications, such as modeling viral emergence, drug-resistance prediction, and therapeutic virus optimization. However, these studies have been mainly performed in cell monolayers, a highly simplified environment, raising concerns about their applicability and relevance. To address this, we compared the evolution of a model virus in monolayers, spheroids, and tissue explants. We performed this analysis in the context of cancer virotherapy by performing serial transfers of an oncolytic vesicular stomatitis virus (VSV-Δ51) in 4T1 mouse mammary tumor cells. We found that VSV-Δ51 gained fitness in each of these three culture systems, and that adaptation to the more complex environments (spheroids or explants) correlated with increased fit

SUBMITTER: Al-Zaher A 

PROVIDER: S-EPMC8134955 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature