Development and validation of a biomonitoring method to measure As, Cr, and Ni in human urine samples by ICP-UCT-MS.

ABSTRACT: We developed an inductively coupled plasma mass spectrometry (ICP-MS) method using Universal Cell Technology (UCT) with a PerkinElmer NexION ICP-MS, to measure arsenic (As), chromium (Cr), and nickel (Ni) in human urine samples. The advancements of the UCT allowed us to expand the calibration range to make the method applicable for both low concentrations of biomonitoring applications and high concentrations that may be observed from acute exposures and emergency response. Our method analyzes As and Ni in kinetic energy discrimination (KED) mode with helium (He) gas, and Cr in dynamic reaction cell (DRC) mode with ammonia (NH₃) gas. The combination of these elements is challenging because a carbon source, ethanol (EtOH), is required for normalization of As ionization in urine sa

SUBMITTER: Jones DR 

PROVIDER: S-EPMC8134962 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature