Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age.

ABSTRACT: Female fertility is inversely correlated with maternal age due to a depletion of the oocyte pool and a reduction in oocyte developmental competence. Few studies have addressed the effect of maternal age on the human mature oocyte (MII) transcriptome, which is established during oocyte growth and maturation, however, the pathways involved remain unclear. Here, we characterize and compare the transcriptomes of a large cohort of fully grown germinal vesicle stage (GV) and in vitro matured (IVM-MII) oocytes from women of varying reproductive age. First, we identified two clusters of cells reflecting the oocyte maturation stage (GV and IVM-MII) with 4445 and 324 putative marker genes, respectively. Furthermore, we identified genes for which transcript representation either progressively increas

SUBMITTER: Llonch S 

PROVIDER: S-EPMC8135014 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature