Peptide location fingerprinting reveals modification-associated biomarker candidates of ageing in human tissue proteomes.

ABSTRACT: Although dysfunctional protein homeostasis (proteostasis) is a key factor in many age-related diseases, the untargeted identification of structurally modified proteins remains challenging. Peptide location fingerprinting is a proteomic analysis technique capable of identifying structural modification-associated differences in mass spectrometry (MS) data sets of complex biological samples. A new webtool (Manchester Peptide Location Fingerprinter), applied to photoaged and intrinsically aged skin proteomes, can relatively quantify peptides and map statistically significant differences to regions within protein structures. New photoageing biomarker candidates were identified in multiple pathways including extracellular matrix organisation (collagens and proteoglycans), protein synthesis and f

SUBMITTER: Ozols M 

PROVIDER: S-EPMC8135079 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature