Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans.

ABSTRACT: Aging leads to a progressive functional decline of the immune system, rendering the elderly increasingly susceptible to disease and infection. The degree to which immune cell senescence contributes to this decline remains unclear, however, since markers that label immune cells with classical features of cellular senescence accurately and comprehensively have not been identified. Using a second-generation fluorogenic substrate for β-galactosidase and multi-parameter flow cytometry, we demonstrate here that peripheral blood mononuclear cells (PBMCs) isolated from healthy humans increasingly display cells with high senescence-associated β-galactosidase (SA-βGal) activity with advancing donor age. The greatest age-associated increases were observed in CD8+ T-cell populations, in which the frac

SUBMITTER: Martinez-Zamudio RI 

PROVIDER: S-EPMC8135084 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature