ChromInst: A single cell sequencing technique to accomplish pre-implantation comprehensive chromosomal screening overnight.

ABSTRACT: Next Generation Sequencing (NGS) is a powerful tool getting into the field of clinical examination. Its preliminary application in pre-implantation comprehensive chromosomal screening (PCCS) of assisted reproduction (test-tube baby) has shown encouraging outcomes that improves the success rate of in vitro fertilization. However, the conventional NGS library construction is time consuming. In addition with the whole genome amplification (WGA) procedure in prior, makes the single cell NGS assay hardly be accomplished within an adequately short turnover time in supporting fresh embryo implantation. In this work, we established a concise single cell sequencing protocol, ChromInst, in which the single cell WGA and NGS library construction were integrated into a two-step PCR procedure of ~ 2.5ho

SUBMITTER: Gao FF 

PROVIDER: S-EPMC8136696 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature