Engineering nucleosomes for generating diverse chromatin assemblies.

ABSTRACT: Structural characterization of chromatin is challenging due to conformational and compositional heterogeneity in vivo and dynamic properties that limit achievable resolution in vitro. Although the maximum resolution for solving structures of large macromolecular assemblies by electron microscopy has recently undergone profound increases, X-ray crystallographic approaches may still offer advantages for certain systems. One such system is compact chromatin, wherein the crystalline state recapitulates the crowded molecular environment within the nucleus. Here we show that nucleosomal constructs with cohesive-ended DNA can be designed that assemble into different types of circular configurations or continuous fibers extending throughout crystals. We demonstrate the utility of the method for ch

SUBMITTER: Adhireksan Z 

PROVIDER: S-EPMC8136823 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature