Berberine regulates lipid metabolism via miR-192 in porcine oocytes matured in vitro.

ABSTRACT:

Background

The berberine (Ber) is an isoquinoline alkaloid compound extracted from Rhizoma coptidis and has the effect that reduces adipose. MicroRNA-192 (miR-192) is related to fat metabolism. However, the relevant mechanism of berberine on lipid metabolism during in vitro maturation (IVM) of porcine oocytes remains unclear.

Objectives

In this study, we investigated the molecular mechanism by which berberine promotes the IVM and lipid metabolism of porcine oocytes via miR-192.

Methods

Ber was added to IVM medium of porcine oocytes. MiR-192 agomir, miR-192 antagomir and negative control fragment were microinjected into the cytoplasm of oocytes without Ber. Rates of oocyte IVM and embryonic development in each group were observed. The content of lipid droplets in IVM o

SUBMITTER: Dai J 

PROVIDER: S-EPMC8136937 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature