Evaluation of a small volume oil-free in vitro production system for bovine embryos.

ABSTRACT:

Background

Embryos are usually produced in culture systems with an oil overlay, which conveys protection against the evaporation of water and microbial contamination. The oil can also release toxic substances and absorb essential components, such as hormones, which adversely affect the quality of the oocytes and the development of embryos in vitro.

Objective

The aim of this study was to validate an oil-free bovine in vitro production (IVP) system.

Method

Cumulus-oocyte complexes collected from abattoir-derived ovaries were matured, fertilized and cultured employing a standard system. The quantity of medium in both groups (with and without an oil overlay) and throughout all stages of IVP was maintained at a volume of 100 μl. The oil group was covered with paraffin oil.

SUBMITTER: Blaschka C 

PROVIDER: S-EPMC8136954 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature