Dataset Information

Standardized and reproducible measurement of decision-making in mice.

ABSTRACT: Progress in science requires standardized assays whose results can be readily shared, compared, and reproduced across laboratories. Reproducibility, however, has been a concern in neuroscience, particularly for measurements of mouse behavior. Here, we show that a standardized task to probe decision-making in mice produces reproducible results across multiple laboratories. We adopted a task for head-fixed mice that assays perceptual and value-based decision making, and we standardized training protocol and experimental hardware, software, and procedures. We trained 140 mice across seven laboratories in three countries, and we collected 5 million mouse choices into a publicly available database. Learning speed was variable across mice and laboratories, but once training was complete there were no significant differences in behavior across laboratories. Mice in different laboratories adopted similar reliance on visual stimuli, on past successes and failures, and on estimates of stimulus prior probability to guide their choices. These results reveal that a complex mouse behavior can be reproduced across multiple laboratories. They establish a standard for reproducible rodent behavior, and provide an unprecedented dataset and open-access tools to study decision-making in mice. More generally, they indicate a path toward achieving reproducibility in neuroscience through collaborative open-science approaches.

SUBMITTER: International Brain Laboratory

PROVIDER: S-EPMC8137147 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Standardized and reproducible measurement of decision-making in mice.

Aguillon-Rodriguez Valeria V Angelaki Dora D Bayer Hannah H Bonacchi Niccolo N Carandini Matteo M Cazettes Fanny F Chapuis Gaelle G Churchland Anne K AK Dan Yang Y Dewitt Eric E Faulkner Mayo M Forrest Hamish H Haetzel Laura L Häusser Michael M Hofer Sonja B SB Hu Fei F Khanal Anup A Krasniak Christopher C Laranjeira Ines I Mainen Zachary F ZF Meijer Guido G Miska Nathaniel J NJ Mrsic-Flogel Thomas D TD Murakami Masayoshi M Noel Jean-Paul JP Pan-Vazquez Alejandro A Rossant Cyrille C Sanders Joshua J Socha Karolina K Terry Rebecca R Urai Anne E AE Vergara Hernando H Wells Miles M Wilson Christian J CJ Witten Ilana B IB Wool Lauren E LE Zador Anthony M AM

eLife 20210520

Progress in science requires standardized assays whose results can be readily shared, compared, and reproduced across laboratories. Reproducibility, however, has been a concern in neuroscience, particularly for measurements of mouse behavior. Here, we show that a standardized task to probe decision-making in mice produces reproducible results across multiple laboratories. We adopted a task for head-fixed mice that assays perceptual and value-based decision making, and we standardized training pr ...[more]

PMID: 34011433

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Standardized and reproducible measurement of decision-making in mice.

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Standardized and reproducible measurement of decision-making in mice.

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