¹⁹F-Tagged metal binding pharmacophores for NMR screening of metalloenzymes.

ABSTRACT: This study demonstrates the screening of a collection of twelve 19F-tagged metal-binding pharmacophores (MBPs) against the Zn(ii)-dependent metalloenzyme human carbonic anhydrase II (hCAII) by 19F NMR. The isomorphous replacement of Zn(ii) by Co(ii) in hCAII produces enhanced sensitivity and reveals the potential of 19F NMR-based techniques for metalloenzyme ligand discovery.

SUBMITTER: Prosser KE 

PROVIDER: S-EPMC8137660 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature