TRPM7 is an essential regulator for volume-sensitive outwardly rectifying anion channel.

ABSTRACT: Animal cells can regulate their volume after swelling by the regulatory volume decrease (RVD) mechanism. In epithelial cells, RVD is attained through KCl release mediated via volume-sensitive outwardly rectifying Cl^- channels (VSOR) and Ca²⁺-activated K⁺ channels. Swelling-induced activation of TRPM7 cation channels leads to Ca²⁺ influx, thereby stimulating the K⁺ channels. Here, we examined whether TRPM7 plays any role in VSOR activation. When TRPM7 was knocked down in human HeLa cells or knocked out in chicken DT40 cells, not only TRPM7 activity and RVD efficacy but also VSOR activity were suppressed. Heterologous expression of TRPM7 in TRPM7-deficient DT40 cells rescued both VSOR activity and RVD, accompanied by an increase in the e

SUBMITTER: Numata T 

PROVIDER: S-EPMC8137958 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature