An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.

ABSTRACT:

Background

Streptococcus pyogenes causes a profound global burden of morbidity and mortality across its diverse clinical spectrum. To support a new controlled human infection ('challenge') model seeking to accelerate S. pyogenes vaccine development, we aimed to develop an accurate and reliable molecular method for quantifying bacterial load from pharyngeal swabs collected during experimental human pharyngitis.

Methods

Combined sequential RNA + DNA extraction from throat swabs was compared to traditional separate RNA-only and DNA-only extractions. An emm-type specific qPCR was developed to detect the emm75 challenge strain. Results from the qPCR were compared to culture, using throat swab samples collected in a human challenge study.

Results

The qPCR was 100% specific

SUBMITTER: Fabri LV 

PROVIDER: S-EPMC8138111 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature