Molecular Characterization of Carbonic Anhydrase II (CA II) and Its Potential Involvement in Regulating Shell Formation in the Pacific Abalone, Haliotis discus hannai.

ABSTRACT: Carbonic anhydrases (CAs) are a family of metalloenzymes that can catalyze the reversible interconversion of CO₂/HCO₃ ^-, ubiquitously present in both prokaryotes and eukaryotes. In the present study, a CA II (designated as HdhCA II) was sequenced and characterized from the mantle tissue of the Pacific abalone. The complete sequence of HdhCA II was 1,169 bp, encoding a polypeptide of 349 amino acids with a NH₂-terminal signal peptide and a CA architectural domain. The predicted protein shared 98.57% and 68.59% sequence identities with CA II of Haliotis gigantea and Haliotis tuberculata, respectively. Two putative N-linked glycosylation motifs and two cysteine residues could potentially form intramolecular disulfide bond

SUBMITTER: Sharker MR 

PROVIDER: S-EPMC8138131 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature