The Universally Conserved ATPase YchF Regulates Translation of Leaderless mRNA in Response to Stress Conditions.

ABSTRACT: The universally conserved P-loop GTPases control diverse cellular processes, like signal transduction, ribosome assembly, cell motility, and intracellular transport and translation. YchF belongs to the Obg-family of P-loop GTPases and is one of the least characterized member of this family. It is unique because it preferentially hydrolyses ATP rather than GTP, but its physiological role is largely unknown. Studies in different organisms including humans suggest a possible role of YchF in regulating the cellular adaptation to stress conditions. In the current study, we explored the role of YchF in the model organism Escherichia coli. By western blot and promoter fusion experiments, we demonstrate that YchF levels decrease during stress conditions or when cells enter stationary phase.

SUBMITTER: Landwehr V 

PROVIDER: S-EPMC8138138 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature