Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.

ABSTRACT: Mobile genetic elements (MGEs) sequester and mobilize antibiotic resistance genes across bacterial genomes. Efficient and reliable identification of such elements is necessary to follow resistance spreading. However, automated tools for MGE identification are missing. Tyrosine recombinase (YR) proteins drive MGE mobilization and could provide markers for MGE detection, but they constitute a diverse family also involved in housekeeping functions. Here, we conducted a comprehensive survey of YRs from bacterial, archaeal, and phage genomes and developed a sequence-based classification system that dissects the characteristics of MGE-borne YRs. We revealed that MGE-related YRs evolved from non-mobile YRs by acquisition of a regulatory arm-binding domain that is essential for their mobility func

SUBMITTER: Smyshlyaev G 

PROVIDER: S-EPMC8138268 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature