A human liver chimeric mouse model for non-alcoholic fatty liver disease.

ABSTRACT:

Background & aims

The accumulation of neutral lipids within hepatocytes underlies non-alcoholic fatty liver disease (NAFLD), which affects a quarter of the world's population and is associated with hepatitis, cirrhosis, and hepatocellular carcinoma. Despite insights gained from both human and animal studies, our understanding of NAFLD pathogenesis remains limited. To better study the molecular changes driving the condition we aimed to generate a humanised NAFLD mouse model.

Methods

We generated TIRF (transgene-free Il2rg ^-/-/Rag2 ^-/-/Fah ^-/-) mice, populated their livers with human hepatocytes, and fed them a Western-type diet for 12 weeks.

Results

Within the same chimeric liver, human hepatocytes developed pronoun

SUBMITTER: Bissig-Choisat B 

PROVIDER: S-EPMC8138774 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature