High glucose treatment promotes extracellular matrix proteome remodeling in Mller glial cells.

ABSTRACT:

Background

The underlying pathomechanisms in diabetic retinopathy (DR) remain incompletely understood. The aim of this study was to add to the current knowledge about the particular role of retinal Mller glial cells (RMG) in the initial processes of DR.

Methods

Applying a quantitative proteomic workflow, we investigated changes of primary porcine RMG under short term high glucose treatment as well as glycolysis inhibition treatment.

Results

We revealed significant changes in RMG proteome primarily in proteins building the extracellular matrix (ECM) indicating fundamental remodeling processes of ECM as novel rapid response to high glucose treatment. Among others, Osteopontin (SPP1) as well as its interacting integrins were significantly downregulated and organotypic re

SUBMITTER: Sagmeister S 

PROVIDER: S-EPMC8139267 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature