Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids.

ABSTRACT: Stem cell technologies including self-assembling 3D tissue models provide access to early human neurodevelopment and fundamental insights into neuropathologies. Gastruloid models have not been used to investigate co-developing central and peripheral neuronal systems with trunk mesendoderm which we achieve here in elongating multi-lineage organized (EMLO) gastruloids. We evaluate EMLOs over a forty-day period, applying immunofluorescence of multi-lineage and functional biomarkers, including day 16 single-cell RNA-Seq, and evaluation of ectodermal and non-ectodermal neural crest cells (NCCs). We identify NCCs that differentiate to form peripheral neurons integrated with an upstream spinal cord region after day 8. This follows initial EMLO polarization events that coordinate with endoderm dif

SUBMITTER: Olmsted ZT 

PROVIDER: S-EPMC8140076 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature