A novel RANKL-targeted flavonoid glycoside prevents osteoporosis through inhibiting NFATc1 and reactive oxygen species.

ABSTRACT:

Background and purpose

Osteoporosis is characterized by excessive bone resorption due to enhanced osteoclast activation. Stimulation of nuclear factor of activated T cells 1 (NFATc1) and accumulation of reactive oxygen species (ROS) are important mechanisms underlying osteoclastogenesis. Robinin (Rob) is a flavonoid glycoside that has shown anti-inflammatory and antioxidative effects in previous studies, but little is known about its effects on bone homeostasis. The purpose of our research was to investigate whether Rob could prevent bone resorption in ovariectomized (OVX) mice by suppressing osteoclast production through its underlying mechanisms.

Methods

The docking pose of Rob and RANKL was identified by protein-ligand molecular docking. Rob was added to bone marrow macro

SUBMITTER: Hong G 

PROVIDER: S-EPMC8140192 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature