Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct Success In Situ.

ABSTRACT: Quantitative diffuse reflectance spectroscopy (DRS) was developed for label-free, noninvasive, and real-time assessment of implanted tissue-engineered devices manufactured from primary human oral keratinocytes (six batches in two 5-patient cohorts). Constructs were implanted in a murine model for 1 and 3 weeks. DRS evaluated construct success in situ using optical absorption (hemoglobin concentration and oxygenation, attributed to revascularization) and optical scattering (attributed to cellular density and layer thickness). Destructive pre- and postimplantation histology distinguished experimental control from stressed constructs, whereas noninvasive preimplantation measures of keratinocyte glucose consumption and residual glucose in spent culture media did not. In constructs impla

SUBMITTER: Lloyd WR 

PROVIDER: S-EPMC8140358 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature