Characterization of full-length transcriptome in Saccharum officinarum and molecular insights into tiller development.

ABSTRACT:

Background

Although extensive breeding efforts are ongoing in sugarcane (Saccharum officinarum L.), the average yield is far below the theoretical potential. Tillering is an important component of sugarcane yield, however, the molecular mechanism underlying tiller development is still elusive. The limited genomic data in sugarcane, particularly due to its complex and large genome, has hindered in-depth molecular studies.

Results

Herein, we generated full-length (FL) transcriptome from developing leaf and tiller bud samples based on PacBio Iso-Seq. In addition, we performed RNA-seq from tiller bud samples at three developmental stages (T0, T1 and T2) to uncover key genes and biological pathways involved in sugarcane tiller development. In total, 30,360 and 20,088 high-quality

SUBMITTER: Yan H 

PROVIDER: S-EPMC8140441 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature